Genistein Induced Apotosis in Human Gastric Carcinoma Cells
The apoptosis in gastric cancer cells induced by genistein and the relationship between this apoptosis and expression of NF-κB and Caspase-3 were studied. In vitro experiments, MTT assay was used to determine the cell growth inhibitory rate. Transmission fluorescence microscope, transmission electron microscopy, DNAfragment assay were used to qualitively detect the apoptosis status of gastric cancer line SGC-7901 before and after the genistein treatment. Immunohistochemical staining and Western Blotting were performed to detect the expression of gene NF-κB and Caspase-3. The results showed that genistein inhibited the growth of gastric carcinoma cell line SGC-7901 in a dose-and timedependent manner. Genistein induced SGC-7901 cells to undergo apoptosis with typically apoptotic characteristics, including morphological changes of chromatin condensation, chromatin crescent formation, nucleus fragmentation and apoptotic body formation. Genistein could reduce the expression of gene NF-κB, and improve the expression of gene Caspase-3.
Ying Liu Xiaomei Li
College of Food Engineering, Harbin University of Commerce, Harbin 150076, China
国际会议
哈尔滨
英文
1283-1286
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）