Sequence length variation of internal genic space of 16S rDNA-23S rDNA in bacterium with high yield of hydrogen production
To develop the identification of species for fermentative biohydrogen-producing bacterium, scholars have found a method which is based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions. In the study, a large fragment of the rDNA operon, including the 16S rDNA, the intergenic spacer region (ISR) and approximately 2000 bases of the 23S rDNA, were polymerasechain reaction (PCR) amplified. The PCR amplification of the genomic DNA of Leptonema ilk strain 3055 using primers directed against conserved regions of the rRNA operon provided evidence that the 16S and 23S rRNA genes were linked via an intergenic spacer region. The sequencing of the intergenic spacer region indicated that it was 435 nucleotides in length and sequence similarity searches revealed that it bore no homology to any known sequences including tRNA available in databases.
Yongfeng Li Yixuan Wang Lu Wang Zhanqing Wang
Forestry School, Northeast Forestry University, Harbin, 150040, P.R.China
国际会议
哈尔滨
英文
1413-1416
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）