Study on the biological activity of anti-tumor peptide of Tumstatin
Tumstatin anti-tumor peptide of 19peptide can inhibit the proliferation of melanoma cell. To study its effect on the proliferation and apoptosis of different tumor cells and verify its anti-tumor non-specificity, the base sequence of 19peptide was designed and constructed engineering bacteria. The soluble 19peptide was obtained from one step chitin affinity chromatograph. By such experiment as MTT assay, cell growth curve, TUNEL assay. flow cytometry, transmission electron microscopy(TEM), the biological activity of 19peptide was studied. Experiments in vitro identified that obtained 19peptide could inhibit proliferation of hela cell and hepatocarcinoma cell. It also could promote two tumor cells apoptosis. Its anti-tumor effect will lay foundation on its mechanism of action research and clinically tumor therapy in future.
Wang Shujing Chen Ning Zhang Yi Liu Jia Xu Beibei
Institute of Pharmacology, Harbin University of Commerce, Harbin 150076, China
国际会议
哈尔滨
英文
1509-1512
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）