Research on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast
The factors of species of the medium, spawn age, species of the enzyme, time of hydrolysis, hydrolysis temperature, osmotic pressure stabilizer and regeneration medium, which have important effects on the Preparation and Regeneration Condition of the Chaetomium cupreum Protoplast, were discussed in this paper. These results showed that the hydrolysis condition can be described as following: Chaetomium cupreum mycelium with spawn age about 48h, the concentration of the lytic enzymes about 2％ and the time of enzymatic hydrolysis 2h. The regeneration condition of the protoplast is that: Chaetomium cupreum mycelium with the spawn age 6h, the concentration of the lytic enzymes 2％, the temperature of 25 and the time of enzymatic hydrolysis 1.5h. We can get the Chaetomium cupreum protoplast, regenerating in the RA Medium with the sorbitol, which regeneration can be over 50％.
Yue hui min Yang qian
School of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China
国际会议
哈尔滨
英文
1522-1526
2011-03-26（万方平台首次上网日期，不代表论文的发表时间）