Genetic variation and phylogenetic relationships of orchardgrass (Dactylis glomerata L.)germplasm assessed by SSR,SRAP,ISSR and AFLP markers
Orchardgrass (Dactylis glomerala L.) is one of the most important cool -season forage grasses, which is widely grown for pasture and hay, owing to its good nutrition, high yield and good tolerance to shadow. SSR, SRAP, ISSR and AFLP molecular markers have been successfully used in assessing genetic diversity and phylogenetic relationships of orchardgrass germplasm origining from 5 continents. High genetic diversity was observed among and within orchardgrass accessions, and analysis of molecular variation (AMOVA) revealed larger genetic variation within accessions and geographical regions rather than between them. Variability within diploid and tetraploid groups was similar, the greater variation was found within ploidy levels rather than between them. Furthermore, the study also indicated that northern Africa, Europe and temperate Asia might be the diversity differentiation center of orchardgrass. The dendrogram based on these markers was concordant with the morphological variability, agronomic traits and karyotype. The result will facilitate the breeding program and germplasm collection and conservation of orchardgrass.
Xinquan Zhang Wengang Xie Yan Peng Linkai Huang Xiao Ma Bing Zeng
Department of Grassland Science,Animal Science and Technology College,Sichuan Agricultural Universit Department of Animal Science,Rongchang Campus of Southwest University,Rongchang 402460,PR China
国际会议
陕西杨凌
英文
69-73
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）