SSR and ISSR Analyses of Genetic Diversity and Relationships in Dactylis glomerata L
SSR (Simple sequence repeat) and ISSR (Inter -simple sequence repeat) molecualr markers were used to evaluate the levels and patterns of genetic diversity among 45 collections of Dactylis glomerata L from four continents. Twenty primer combinations were used and 295 bands were produced in SSR, of which 187(61.15%) were polymorphic. On the other hand, twelve primers were used to generate a total of 116 bands in ISSR, of which 101(87.07%) were polymorphic. The coefficient range of genetic similarity was 0.7848-0.9513 and 0.6116-0.9231 respectively. Based on cluster and principal component analysis on the generic characteristics, all collections could be divided into six groups and five groups in two markers, respectively. According to the analysis of genetic diversity and relationships, the appropriate strategies for collection and conservation of germplasm resources also were discussed and scientific breeding with far generic relationship materials in Dactylis glomerata L were suggested.
Bing Zeng Fuyuan Zuo Xinquan Zhang Wengang Xie
Department of Animal Science, Southwest University(Rongchang Campus),Chongqing,402460,PR China Department of Animal Science,Southwest University(Rongchang Campus),Chongqing,402460,PR China Department of Grassland Science,Sichuan Agricultural University,Yaan 625014,PR China
国际会议
陕西杨凌
英文
104-106
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）