Gliadin analysis on genetic diversity and genetic structure of wild Elymus nutans Griseb.germplasm from western China
Elymus nutans Griseb. As an important forage grasses, which is widely grown in alpine meadow, owing to its high adaptability, good nutrition, high yield and good tolerance to cold, drought and pest. Acid polyacrylamide gel electrophoresis (A-PAGE) was used to assess the gliadin genetic diversity and generic structure of 62 wild accessions. A total of 42 bands were detected in all accessions, 92.86% were polymorphic bands. The Neis genetic similarity coefficient ranged from 0.457 to 1.000 and with an average of 0.759. 62 accessions can be clustered into 3 groups at GS=0. 736 level on dendrogram. The principal coordinates analysis (PCoA) reflected almost the same relationships among the studied materials as shown in cluster analysis. The findings implied a correlation between gliadin patterns and eco -geographical environment. Genetic diferentiation among and within six geographical groups was estimated by Shannon diversity index, which revealed that 48. 78% and 51. 52% variation accounted for among and within the groups. Based on Neis unbiased measures of genetic identity, UPGMA cluster analysis measures of six geographical groups indicated that there was a correlation between genetic diferentiation and geographical habits among the groups.
Jiamin Miao Xinquan Zhang Shiyong Chen Xiao Ma Zhihua Chen Shiqie Bai
Department of Grassland Science,Animal Science and Technology College,Sichuan Agricultural Universit Life Science and Technology ollege,Southwest University for Nationalities,Chengdu 610041,PR China Sichuan Grassland Science Academy,Chengdu 611731,PR China
国际会议
陕西杨凌
英文
107-111
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）