A 4CL—like Gene from Galega orientalis was up—regulated by variety abiotic stress
Lignin is an important biopolymer in higher plants and plays an important role in growth and development of plants. 4 -coumarate;CoA: Aligase (4CL) gene encode a key enzyme in biosynthesis process of lignin. Galega orientalis is a promising perennial nitrogen -fixing forage legume for forage production and soil improvement, possessing strong drought and cold resistance. Using RACE technology and RT -PCR method, a full length cDNA, which encode a 4CL -gene, was isolated from G.. Orientalis, termed Go4CL. Drought stress significantly induced Go4CL gene expression. Exogenous phytohormones, such as ABA, MeJA and SA, all up -regulate the gene expression of Go4CL. These results indicated that GcACL maybe involved in variety of stress -resistance regulation pathway. Isolation and characterization of 4CL genes from G. Orientalis would help us to understand its excellent resistances to environmental stresses and lay a foundation for genetic improving stress tolerance in pasture or other crops.
Xuemin Wang Dongmei Yang Zan Wang Hongwen Gao
Institute of Animal Science,Chinese Academy of Agriculture Sciences,Beijing,100193,China
国际会议
陕西杨凌
英文
169-173
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）