Title: Verification of specific G-quadruplex structure by using a novel cyanine dye supramolecular assembly: Ⅱ. The binding characterization with specific intramolecular G-quadruplex and the recognizing mechanism

摘要：

The supramolecular assembly of a novel cyanine dye, 3,3-di(3-sulfopropyl)-4,5,4,5-dibenzo-9-ethylthiacarbocyanine triethylammonium salt (ETC) was designed to verify specific intramolecular Gquadruplexes from duplex and single-strand DNAs. Spectral results have shown that ETC presented two major distinct signatures with specific intramolecular G-quadruplexes in vitro: 1) dramatic changes in the absorption spectra (including disappearance of absorption peak around 660 nm and appearance of independent new peak around 584 nm); 2) about 70 times enhancement of fluorescence signal at 600 nm. Furthermore, based on H-NMR and molecular modeling (Insight II software), the preferring binding of ETC to specific intramolecular Gquadruplexes probably result from end-stacking, and the loop structure nearby also plays an important role.

作者: Qianfan Yang Guangzhi Xu Junfeng Xiang Shu Yang Qian Li Qiuju Zhou Aijiao Guan Xiufeng Zhang Hong Zhang Yalin Tang

作者单位: Beijing National Laboratory for Molecular Sciences (BNLMS), Center for Molecular Sciences, State Key Beijing National Laboratory for Molecular Sciences (BNLMS), Center for Molecular Sciences, State Key

会议类型: 国际会议

会议名称: The 5th International Conference of Molecular Simulations and Applied Informatics Technologies(第五届国际分子模拟与信息技术应用学术会议 ICMS&I)

会议地点: 武汉

会议语种:英文

页码: 349-377

在线出版日期: 2010-09-01（万方平台首次上网日期，不代表论文的发表时间）