Relationships between Inhibition Constants, Types of Inhibition and IC50 Calculation and Comparison on IC50 of Two Model Tyrosinase Inhibitors

摘要：

Calculating methods for inhibitor concentration (IC50) leading to 50% activity lost (for diphenolase activity) of a novel parabolic competitive inhibitor (the trifluoromethyl-containing 1,2,3-triazole, TF-TA) and a novel mixed inhibitor (E-4-(1-imidazoylmethyl) cinnamic acid (ozagrel) were derived on the basis of kinetics of tyrosinase inhibition. These methods were applied to calculate IC50 values of the two tyrosinase inhibiting compounds. The experimental IC50 values of TF-TA and ozagrel were 41.9 μM and 3.45 mM, respectiviely. Moreover, the calculated IC50 value of TF-TA and ozagrel were 82.8 μM and 3.15 mM, respectively. The calculation result of ozagrel is closer to experimental data.

作者: Hairong Mao Jiangang Xie Qimeng Zhang Xinyu Lü Shubai Li

作者单位: Department of Chemistry, Zhengzhou Teachers College, Zhengzhou, China Department of Chemical Engineering Technology, Changzhou Institute of Engineering Technology, Changz Institute of Fine Chemicals, Jiangsu Polytechnic University, Changzhou, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）