Application of Agilent 2100 Bioanalyzer in Multiplex PCR for Detection of Foodborne Pathogens

摘要：

To explore the feasibility of using Agilent 2100 Bioanalyzer in the detection of the three kinds of food-borne pathogens, three pairs of primers were designed according to invA gene of Salmonella ipah gene of Shigella and inv gene of Yersinia pseudotuberculosis, and then the single PCR was performed to detect specificity. Those genes were amplified by Multiplex PCR technique using specific primers, and the optimum of multiplex PCR reaction conditions. The multiplex PCR detection method which provided a basis for further research on developing the food-borne pathogens detection kit was very practical. Agilent 2100 Bioanalyzer combined with Multiplex PCR was specific, accurate and sensitive for foodborne pathogens Salmonella, Shigella and Yersinia pseudotuberculosis detection.

作者: WANG Bo-fei BAI Yan-hong ZHAO Pei-pei WANG Yun-long JING Jian-zhou

作者单位: College of Food Biotechnology Engineer, P.R Zheng Zhou University of Light Industry, Zhengzhou Henan Henan Bioengineering Technology Research Center, Zhengzhou Henan 450002, P.R China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）