MDM2 gene promoter polymorphism and risk of cervical cancer in Chinese population

摘要：

Purpose: Mouse double minute 2 (MDM2) is a key negative regulator of the p53 activity. Recently, a polymorphism in the intronic promoter, SNP309, was shown to influence MDM2 expression and p53 activity. We examined whether the SNP309 was related to the risk of developing cervical cancer among Chinese populations. Experimental Design: We genotyped the MDM2 polymorphism SNP309 of peripheral blood DNA from 110 cervical cancer patients and from 160 controls. Genotyping were confirmed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing. The logistic regression analysis was used to calculate adjusted odds ratio (OR) and 95% confidence interval (95%CI). Results: We observed that compared with the TT genotype, the GG genotype was associated with significant increased susceptibility to cervical cancer (OR=2.69, 95% CI=1.26-5.71, P=0.008). The relative frequency of each allele was 0.623 for G and 0.377 for T in patients with cervical cancer, and 0.50 for G and 0.50 for T in normal controls (P<0.05). Furthermore, we also found that the MDM2 SNP309 GG genotype was significantly associated with lymph node metastasis but not tumor histological type, tumor grade and size. Conclusions: Our findings show a significant association between functional polymorphism in MDM2 and increased risk of developing cervical cancer in Chinese population. In addition, the SNP309 GG genotype may be a risk factor for lymph node metastasis of cervical cancer.

作者: Pei Jiang Jianxin Liu Xiaoxi Zeng Wen Li Jianxin Tang

作者单位: School of Info-physics and Geomatics Engineering Central South University ChangSha 410083, China Gre School of Info-physics and Geomatics Engineering Central South University ChangSha 410083, China Green Packaging and Biological Nanotechnology Laboratory Hunan University of Technology Zhuzhou 4120 School of Info-physics and Geomatics Engineering Central South University ChangSha 410083, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）