Identification of a novel S-RNase gene and Sgenotypes of four pear (Pyrus pyrifolia) cultivars
Gametophytic self-incompatibility (GSI) is a genetically controlled mechanism to prevent inbreeding and promote out-crossing. Pyrus pyrifolia is a commercially important fruit tree that exhibits GSI. It is necessary to identify S-genotypes of cultivars for determination of cross-compatible combination prior to performing pear plantation and breeding programs. In this study, an important local cultivar ‘Huobali’ extensively used as parent in pear breeding programs, and its three progenies ‘Mantianhong’, ‘Hongsucui’ and ‘Meirensu’ were used for Sgenotyping analysis by PCR-based molecular method. Agarose gel electrophoresis showed that two fragments were produced in each of the three progenies, while three in the ‘Huobali’. After restriction digestion and sequencing analysis, the S-alleles in the four cultivars were determined. The allele of 345 bp from ‘Huobali’ was determined as a novel S-RNase allele that was tentatively denominated as S44-RNase. RT-PCR revealed that the S44-RNase was expressed specifically in the styles, which is consistent with the expression pattern of S-RNases. Comparison of genomic and cDNA sequences revealed an intron of 148 bp for the S44-RNase. At the amino acid level, it shared 65% to 93% similarity with other Maloideae S-RNases. This study will be helpful in pear production and breeding programs.
Zhang Lin Hu Jiao Tan Xiaofeng Long Hongxu Yuan Deyi Li Xiugen
The Key Lab of Non-wood Forest Product of Forestry Ministry, College of Forestry,Central South Unive Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou city, 450009
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）