Cloning and Sequence Analysis of a cDNA of P450 17α-hydroxylase/17,20-lyase(CYP17) from Strongylocentrotus Nudus

摘要：

CYP17 (17α-hydroxylase/17, 20-lyase) has been viewed as a critical enzyme for the biosynthesis of gonadal and adrenal steroids in vertebrates. According to a reported CYP17 gene of Strongylocentrotus purpuratus gene from NCBI, three pairs of primers were designed to clone CYP17 gene of Strongylocentrotus nudus,using RT-PCR. Three fragments were assembled into on contig of 1569bp, including full CDS of 1482bp encoding 493 amino acid residues. Sequence analysis indicated the two sea urchin CYP17 genes were mostly similar(97%). But their amino acid identities were low compared with vertebrates. Cloning of this CYP17 gene would be necessary to characterize its function in development of gonads of sea urchin.

作者: Xiaoli Guo Yang Liu Xuemei Qiu Xiuli Wang

作者单位: College of Life Sciences and Biotechnology,Dalian Fisheries University, Dalian 116023, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）