Comparison of eleven single-round and multiple PCR assays targeting IS6110, IS1081,recA intein and pps1 intein gene for detection of Mycobacterium tuberculosis

摘要：

For molecular biological detection of Mycobacterium tuberculosis, PCR methods with primers targeting different regions specific for mycobacterium tuberculosis complex are used worldwide. However, some uncertainties exist concerning the specificity of certain target regions and the sensitivity. To identify the methods which are best suited for diagnostics, 9 single-round and 2 multiple PCR systems based on IS6110 , IS1081, recA intein, and pps1 intein gene sequences were compared regarding their analytical sensitivity and specificity under similar PCR conditions.

作者: Chun-Fang Wang Xiu-Yun Jiang Yun-Hang Gao Gong-Mei Li Hai-Yong Guo Hao-Wang Ai-dong Qian

作者单位: Animal Science College Jilin Agricultural University Changchun,China Key Laboratory of Animal Produc College of Life Sciences Jilin Agricultural University Changchun,China Animal Science College Jilin College of Life Sciences Jilin Agricultural University Changchun,China Animal Science College Jili

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-5

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）