Construction of a New Universal PlantOverexpression Vector for cDNA Transformation

摘要：

The transformation of cDNA into plant is still not carried out directly by a universal transgenic vector, hence it is not convenient for the transgenic application of those functionimportant cDNAs. Herein we introduced a rapid, cost-effective PCR-based DNA synthesis method to construct a new universal plant overexpression vector for the direct inserting of target cDNA during transgenic application. The new engineered transgenic vector was based on the pCAMBIA vector backbone, which was inserted by five motifs, including a promoter, an enhancer, a Linker DNA fragment, a endoplasmic reticulum retention signal (KDEL), and a NOS-T DNA. The target cDNA can be directly inserted into the new universal vector to construct the workable transgenic plasmid according to the interesting need of transgenic users. The result reported here will promote the plant transgenic application of cDNAs.

作者: Dang-Quan ZHANG Zhen-Jun GU Shun-Yang DENG Shao-Gang FAN Quan-Dong ZHU

作者单位: Biotechnology Core Facilities/Key Lab of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology, Changsha 410004, P.R.China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）