Prediction of B Cell Epitopes and Overexpression of Truncated VP19c of Duck Enteritis Virus in Escherichia coli

摘要：

Prediction of B cell epitopes of duck enteritis virus (DEV) VP19c protein encoded by UL38 gene and expression in prokaryotic system were described in the present study. The major antigenic portion of VP19c protein of DEV was predicted and corresponding gene was amplified from the extracted DNA and cloned. The recombinant expression construction(pPAL7-truncated UL38) was identified by the polymerase chain reaction, restriction enzyme and sequencing analysis. The profinity eXact affinity-tagged fusion truncated VP19c protein with a molecular mass of 35 kDa was expressed by IPTG induction and was purified to near homogeneity by extracting inclusion bodies(IBs) as judged by sodium dodesyl sulfate-polyacrylamide gel electrophoresis analysis. The purified truncated VP19c protein was recognized by antibody to DEV in western blot analysis.

作者: JunXiang Qihui Luo Hengmin Cui Yi Zhou YinWang Zhiwen Xu Zhengli Chen Xiaoyue Chen Xiaoyu Wang Anchun Cheng Xiaoyue Chen Anchun Cheng Mingshu Wang HuaChang Shunchuan Zhang Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia

作者单位: Avian Diseases Research Center College of Veterinary Medicine of Sichuan Agricultural University Yaa Key Laboratory of Animal Diseases and Human Health of Sichuan Province Yaan, Sichuan, 625014,China Epizootic Diseases Institute of Sichuan Agricultural University Ya’an, Sichuan, 625014, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-5

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）