Disruption of PDC1 Gene to Enhance Pyruvic Acid Accumulation of Saccharomyces cerevisiae Subtitle: PDC1 disruption for pyruvate accumulatin of S. cerevisiae

摘要：

The PDC1 gene encoding a pyruvate decarboxylase in S. cerevisiae and Kanr gene were respectively amplified by PCR using oligonucleotides which contained the restriction of NotI, EcoRI, and BglII. The two genes were digested with the same EcoRI and BglII, and linked together for inserting the Kanr gene into the PDC1 gene while the gene disruption cassette was constructed. After transformation of the linear disruption cassette (P1K) into S. cerevisiae Y2, selected transformants were checked by PCR for correct recombination. The haploid mutant Y2-1△ was obtained, which could increase the yield of pyruvic acid by 176% by shaking flask cultivation as compared with the parental strain Y2.

作者: WANG De-pei DING Xin DENG Xu-heng ZHAO Qin GAO Qiang GAO Nian-fa

作者单位: School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R.China Key School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R.China Tia

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-3

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）