Heterologous expression of ksdD gene encoding 3-ketosteroid-△1-dehydrogenase from Arthrobacter simplex in Escherichia coli

摘要：

Arthrobacter simplex is extensively used in pharmaceutical industry to introduce C1-C2 double bond into steroid nucleus. This dehydrogenation reaction is known characterized by its 3-ketosteroid-△1-dehydrogenase(KSDH), which is encoded by ksdD gene. In this research, we report the expression of KSDH in Escherichia coli. The activity of the enzyme has been determined by spectrophotometric method. We found the activity of purified enzyme was about 5 fold more than the crude enzyme of cell extract. Additionally, compared with A. simplex, the transformation level by broken cell preparations of E. coli recombination cells used in the dehydrogenation reaction of 4-androstene-3, 17-dione was dramatically improved.

作者: Yu Li Dong-jun Kong Fu-ping Lu Hong-hong Jia Jiang Li Yue-ting Cao

作者单位: Key Laboratory of Industrial Microbiology, Ministry of Education,College of Biotechnology, Tianjin U Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-5

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）