Listeria monocytogenes Sigma B contributes to the tolerance of bile stress in the gastrointestinal tract
The ability of Listeria monocytogenes resisting bile stress is very important for its successful infection and colonization in the human gastrointestinal tract. Some studies have indicated that the alternative sigma factor Sigma B (σB), encoded by sigB, is at least partly involved in bacteria resisting many adverse conditions. In order to investigate the role of L. monocytogenes Sigma B in bile tolerance in the gastrointestinal tract, sigB mutant strain EGDΔsigB was constructed, and in parallel with wild type strain EGD, exposed to 3% bile salt(sublethal concentration for bacteria) with or without preexposure to acid (pH 4.5), alkali (pH 9.0), or osmotic (0.3M NaCl) conditions by mimicking the human gastrointestinal juice for 30 min. Their growth and survival rates were examined after 5 hours cultivation. The results were shown (ⅰ) wild type strain EGD was more resistant to 3% bile salt stress than sigB mutant strain EGDΔsigB; (Ⅱ) Both alkali-and osmotic-adapted EGD and EGDΔsigB were more resistant to 3% bile salt than nonadapted strains; (iii) acid-adapted EGD and EGDΔsigB didn’t increase their ability to resist 3% bile salt. These data indicate that L. monocytogenes σB is required for bile tolerance in the gastrointestinal tract, and also reveal some σB-dependent crossprotection mechanisms involving in tolerance of alkaline (or osmolality) and bile salt stress.
YINGYING FENG QIANG ZHANG LI WANG FEIFEI FENG QIN LUO
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）