Gene Expression Profiling of Idiopathic Orbital Inflammatory Pseudotumors
Propose: Idiopathic orbital inflammatory pseudotumors (IOIP) are inflammatory pseudotumors (IPT) unknown etiology that develop in the orbit. Due to the lack well-defined pathogenic mechanisms and diagnostic markers, diagnosis and treatment of this disease remain a significant challenge. Therefore, the aims of this study are to define the etiological factors of IOIP and to identify potential gene targets for the treatment of IOIP. Methods: Eleven IOIP tissue samples were obtained from patients during surgical resection; as control tissue, four normal tissue samples were collected from surgery removal of eye due trauma. Total RNA was extracted and the gene expression profile of both diseased and normal tissues was analyzed using human oligonucleotide microarrays, which contained 32,050 wellcharacterized human genes. The expression patterns of selected genes were further confirmed by Real-time RT-PCR. Results: Microarray analyses identified 744 genes differentially expressed in IOIP and non-IOIP samples, with at least 21.5-fold changes and P-value <.005, including 552 downregulated and 192 upregulated genes in IOIPs. Principal Component Analysis (PCA) and Hierarchical Clustering of microarray data successfully distinguished the IOIP group from the non-IOIP control group. Functional annotation using the Molecule Annotation System categorized the 744 genes into immune functions, inflammatory responses, signaling molecules in the PI3K and NF-κB pathways, extracellular matrix degradation, and unknown functions. Conclusions: Immune and inflammatory responses and activation of the PI3K and NF-κB pathways are likely associated with IOIPs. Drugs that would suppress these two responses and two signal pathways may offer new therapeutics of IOIP treatment.
Rui Jin Xin Ge Xue-mei Ma Peng-xiang Zhao Juan Feng Li-ning Guo Ru-gang Zhong Yi Zeng Jian-min Ma Ji-tong Shi
College of Life Science and Bio-engineering,Beijing University of Technology,Beijing 100124, P.R.Chi Beijing Tongren Ophthalmic center, Beijing Tongren Hospital,Capital Medical University,Beijing 1007 College of Life Science and Bio-engineering,Beijing University of Technology,Beijing 100124, P.R.Chi Beijing Tongren Ophthalmic center, Beijing Tongren Hospital,Capital Medical University,Beijing 10073
国际会议
成都
英文
1-6
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）