Bioinformatics Analysis and Characteristics of Glycoprotein K Encoded by the Newly Identified UL53 Gene of Duck Enteritis Virus
Glycoprotein K encoded by the DEV-UL53 gene is one of the DEV envelope glycoproteins. Like most members of the alphaherpesvirinae, the genes in the UL region of its genome are well conserved, which means that the major DEV envelope glycoprotein K possessing similar functions of envelope glycoprotein K of alphaherpesvirinae plays an essential role in viral replication and cell fusion. Glycoprotein K sequence analysis indicates that the protein possesses typical characteristics of membrane glycoproteins containing five transmembrane helix structures and an NH2-terminal signal peptide cleavage site, which provide some information for its expression. The precursor gK has five hydrophobic domains, which consists of 343 amino acids and exhibits a molecular mass of 38.12 kDa. Through combining the result of estimating potential antigenic epitopes and secondary structure presumption, five B cell dominant epitopes can be initially definited, which locate at 60-62, 144-155, 157-160, 210-214 and 293-294 amino acids. In conclusion ，all the data and consequences will provide a basis for further functional study of the DEV-glycoprotein K and provide necessary data for the new type clinical diagnosis of DEV and the development of new DEV vaccine.
Shunchuan Zhang Hengmin Cui Yi Zhou Yin Wang Zhiwen Xu Zhengli Chen Xiaoyue Chen Xiaoyu Wang Anchun Cheng Xiaoyue Chen Anchun Cheng Mingshu Wang Jun Xiang Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Qihui Luo
Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Ya’a Key Laboratory of Animal Disease and Human Health of Sichuan Province,Sichuan Agricultural Universit Epizootic Diseases Institute of Sichuan Agricultural University Ya’an, Sichuan, 625014, China
国际会议
成都
英文
1-8
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）