Molecular Characterization Analysis of Newlyidentified Duck Enteritis Virus UL55 Gene

摘要：

The focus of this paper is on the newly identified UL55 gene of DEV in our laboratory.Available bioinformatic tools were used to describe its molecular characteristics. A identified nucleotide sequence about 561bp of DEV UL55 ,consisting a complete ORF, was deduced to encode a protein with 186 amino acids.The 20.7981 kDa protein whose singnal peptide and transmembrane region are both absent. Codon usage analysis shows the gene could be well expressed in Escherichia BL21.The putative protein is hydrophilic on the whole,its antigen epitope centralizes at the Extended strand (Ee) and Random coil (Cc) regions as well as the corresponding hydrophilic regions.It was predicted to be a nucleoprotein, which is probably invovled in viron entry,assembly,egress,maturation and release as its homologous.However, UL55 protein just plays an accessory role. Phylogenetic tree based on amino acids and its reference herpesvirus reaveals that there is a close relationship between DEV and Mardivirus genus.However, DEV is clustered within a monophyletic clade among others , suggests it is an intermediate taxonomic entity and should be placed in a single genus within the herpetoviridae subfamily.Despite its preliminary characterization, this study can clearly direct our research to elucidate its biological function and physiological features of this newly identified UL55 gene.

作者: Ying Wu Zhengli Chen Xiaoyue Chen Anchun Cheng Mingshu Wang Dekang Zhu Renyong Jia Hengmin Cui Qihui Luo Yi Wang Zhiwen Xu

作者单位: Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Ya’a Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural Universi Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural Univers Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Ya’a Avian Disease Research Center College of Veterinary Medicine of Sichuan Agricultural University Ya’a

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-7

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）