Purification and characterization of cathepsins from the gut of apostichopus japonicus
Cathepsins from gut of apostichopus japonicus were prepared by ammonium sulfate fractionation, purified by DEAE-52 and Sephadex-100 chromatographic separations. cathepsins were purified more than 66 folds and yield reduced to 1.7%. Optimal pH and temperature were 7.0 and 35℃, respectively.
Bing Han Xue Han Beiwei Zhu
Collage of Food Science and Engineering, Harbin University of Commerce, Harbin 150076, P.R.China College of Landscape Architecture, Northeast Forestry University, Harbin 150040, P.R.China School of Food Biological & Food Engineering, Dalian Polytechnic University, Dalian 160034, P.R.Chin
国际会议
成都
英文
1-3
2010-06-18(万方平台首次上网日期,不代表论文的发表时间)