Acetone extracts of sea urchin Anthocidaris crassispina stimulate osteogenesis and adipogenesis in mouse mesenchymal stem cells
In this study, it was found that the acetoneextracts of Anthocidaris crassispina could stimulate bothosteogenesis and adipogenesis in mouse mesenchymal stem cells(MSCs). The sea urchin was disparted into three parts: shell,spindle and gonad (Sh, Sp and G in short, respectively), andextracted with acetone. Either with or without inducers, Sp andG at concentrations of 0.2 or 0.5 mg/ml remarkably stimulatedthe ALP activity and the formation of fat droplets in MSCosteogenesis and adipogenesis, respectively. However, Shdisplayed no significant effect on either of the differentiations.Moreover, the expression levels of Runx2 and PPARγ2 were alsoup-regulated upon Sp and G treatments significantly, but notwith Sh treatment. Our study demonstrated for the first time thatthe Sp and G from A. crassispina may be beneficial in theprevention of some bone related diseases and insulin resistancevia its potency to stimulate osteogenesis and adipogenesis ofMSCs.
Hai-fang Li Guo-ping Cai Hai-fang Li Meng-su Yang
Department of Biological Sciences and Biotechnology,Tsinghua University,Beijing, China, 100084 Shenzhen Key laboratory of Biochip Research,City University of Hong Kong,Shenzhen, China, 518057
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）