Repair of Deep Burn Skin Defects with Cultured Epidermal Stem Cells and Acellular Amniotic Membranes
Tissue engineered skin constructed by culturedseed cells provides a new way to accelerate skin wound healingand improve the quality of tissue repair. Conventional modelswith dermal scaffold material based on collagen hydrogels,however, have poor stability and unsatisfactory longevity.Here, we describe an improved acellular amniotic membraneas a scafford with epidermal stem cells for tissue engineeringskins and observe their clinical effect on deep burn wounds.The human epidermal stem cells was isolated from the skinsamples by trypsin digesting method and purified by collagenadhering method, and then seeded on the acellular amnioticmembrane to form the engineered skin. The biologicalattachment and growth of cultured epidermal stem cells wereobserved. Twelve cases of deep burn wounds were applied withthe engineered skin. The results showed that the epidermalstem cells adhered to the surface of acellular amnioticmembrane quickly after being seeded and exhibited a highcolony formation efficiency. The wounds treated withengineered skin healed rapidly with good clinical take. Theaverage time of wound closure in engineered skin group wasshortened than that of control group. There were no obviousevidence of immunological rejection and inflammatoryreaction during the observation. This indicated that theacellular amniotic membrane with good histocompatibilitymay be a suitable scaffold with epidermal stem cells in theconstruction of tissue engineering skin to repair deep burnwounds.
Dewu Liu Honglan Xiong Yuangui Mao Peixin Huang Jianping Chen Wei Lan
First Affiliated Hospital Nanchang University Nanchang, Jiangxi Province, P.R.China
国际会议
成都
英文
1-3
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）