Degradation of hemicelluloses of cottonseed coat by xylanase

摘要：

The hemicelluloses were separated from the cottonseedcoat and degraded with Pulpzyme HC. The digestion degreevalue was calculated by the equation depended on the release ofreducing sugars concentration which was determined from DNSfitting curve equation. The optimum enzyme reactiontemperature was 60 ℃and pH was 5.5. The duration of 80 minwas chose as the most suitable enzymolysis time. Thedegradation degree value was 57.5% when 0.24 ml purePulpzyme HC was added with one milligramme hemicellulosespowder. The K+content substrate (0.5 mmol L -1) enhanced theenzymic degradation of hemicelluloses. The other metal ionswere found to be some inhibitors of Pulpzyme HC. The effects ofchelating agent (EDTA) were complex and no trend wasobserved.

作者: Ying-qing Hu Hua-Li Nie Li-Min Zhu Christopher J Branford-White

作者单位: College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, P.R.Chin College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, P.R.Chin Institute for Health Research and Policy London Metropolitan University London, UK

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）