Extraction of mycelial polysaccharides from submerged-cultured Agaricus blazei

摘要：

Aqueous extraction and alkaline (NaOH) hydrolysismethod were comparatively studied in polysaccharides extractionfrom submerged-cultured Agaricus blazei mycelia. The resultsshow that the polysaccharide content and the total extract ratefrom alkaline extraction method were obviously higher thanthose from the aqueous extraction method. Optimization resultsby the orthogonal matrix method on polysaccharide extractionfrom A. blazei show that the optimum alkaline concentration was1.0 mol/L, hydrolysis temperature 60 ℃, hydrolysis time 1 hour,and liquid: solid ratio 15. With the optimized condition, thepolysaccharide extraction rate reached 11.93 ± 0.68 %. Themycelial polysaccharide obtained with alkaline hydrolysismethod was fractionated by the DEAE column chromatography.Five APS fractions were isolated in the polysaccharide samplewith APS-1 as the main component.

作者: Xiao-Ling Wang Gao-Qiang Liu Huai-Yun Zhang Guo-Ying Zhou

作者单位: Core Facilities of Biotechnology, Central South University of Forestry and Technology Changsha 410004, P.R.China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）