Intragastric administration of Lactobacillus casei expressing bovine rotavirus VP7 protein induced specific antibody production
The aim of this study is to explore the mucosal andsystemic antibody immune responses induced by recombinantL.casei expressing the major immunoprotective antigen VP7 ofbovine rotavirus (BRV). Two inducible expression vectorscontaining the antigenic dominant region of VP7 gene, pPG-1-VP7(cell-surface expression vector) and pPG-2-VP7 (secretoryvector), were constructed and electrotransformed into L. casei393 to obtain positive recombinant bacteria designatedrLc::pPG-1-VP7 or rLc::pPG-2-VP7. The expressions of VP7protein induced by 2% lactose in MRS media were confirmed bywestern blot and indirect immunofluorescent staining using anti-VP7antibody. Oral immunization of mice with the recombinantstrains significantly increased anti-VP7 sIgA level in mousevaginal lavages and feces samples, and anti-VP7 IgG level inserum compared with empty plasmid transformed bacteria.Results of neutralization assay clearly indicated that serumantibodies induced by oral vaccination have neutralizationactivity against BRV in vitro. The neutralizing titers are 1:62.9and 1:50.6 for rLc::pPG-1-VP7 and rLc::pPG-2-VP7,respectively. Our results demonstrated that Lactobacillus caseiexpressing rBRV-VP7 have application potentials in producingoral vaccine against BRV.
Songmei Liu Didi Liu Guanqun Zhang Yijing Li
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China Key Laborato College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China
国际会议
成都
英文
1-5
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）