Synthesis of functional feruloylated lipids through enzymatic irreversible transesterification protocols
Functional feruloylated lipids, which are composed of1(3)-feruloyl-monooleyl-glycerol (FMOG) and 1(3)-feruloyl-dioleyl-glycerol (FDOG), was prepared through the lipase-catalyzedirreversible transesterification between vinyl feruate(VF) and triolein (TO) in toluene. The nuclear magneticresonance (NMR) and electrospray ionization-mass spectroscopy(ESI-MS) analysis confirmed the structure of VF. The influencesof molar ratio of substrates, reaction time, reaction temperature,water activity and enzyme amount on the total conversion ofreaction and conversion yield of FMOG and FDOG wereanalyzed systematically. The optimal conditions are as follows:VF:EF=1:3, 62h, 55℃, aw 0.07, 20mg/mL Novozym 435. Underthe optimal conditions, the average highest conversion offeruloylated lipids achieved was 92.29±2.13%, which wascomposed of FMOG 39.78±4.85% and FDOG 52.51±6.01%,respectively. It could provide an efficient approach for preparingferuloylated lipids.
Yang Yu Yan Zheng Jing Quan Ya-Juan Wang Li-Min Zhu Christopher J Branford-White
College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,Shanghai, P.R.China College of Chemistry, Chemical Engineering and Biotechnology, Donghua University,Shanghai, P.R.China Institute for Health Research and Policy London Metropolitan University, London, UK
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）