Biotransformation of DL-2-Amino-?2 -Thiazoline--4-Carbonic Acid to L-Cysteine Using a Newly Isolated Pseudomonas sp. Zjwp-14

摘要：

A bacterial strain Pseudomonas sp. Zjwp-14 wasscreened out from soil, and used for the biotransformation ofDL-2-amino-?2 -thiazoline-4-carbonic acid to L-cysteine. Thisstrain was identified based on its morphological, physiologicalcharacterization, 16S rDNA sequence determination andphylogenetic analysis. The bioconversion process was optimizedby examining some key factors including cell cultivation time,buffer pH, metal ions, aeration (anaerobic/aerobic) and substrateunder optimal conditions: cells were cultivated for 12 h, and thenincubated in still culture flashing with N2 atmosphere at 42 o C for6 h. The results suggested that Pseudomonas sp. Zjwp-14, abacterial isolate, has applied potential for enzymatic productionof L-cysteine.

作者: Pu Wang Guoying Lv Junyao He Wei qing Chen

作者单位: College of Pharmaceutical Science Zhejiang University of Technology, 310032,Hangzhou, P.R.China College of Biological and Environmental Engineering Zhejiang Shuren University, 310015,Hangzhou, P.R

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）