Acellular tissue engineering scaffolds for vocal fold regeneration
A variety of xenogeneic and allogeneic extracellularmatrix (ECM) scaffolds have been promising in various tissueengineering applications, such as the porcine small intestinalsubmucosa. Yet no particular scaffolds have been optimal forvocal fold regeneration. The decellularized human umbilical vein(HUV) is a novel allogeneic scaffold that has shown some promisefor cardiovascular tissue engineering. This study examines thepotential of the HUV as an acellular scaffold for engineering thevocal fold lamina propria, in order to develop an implantabletissue substitute that can promote a natural ECM remodelingresponse. A novel saline-based decellularization protocoldeveloped in our laboratory was used to fabricate a three-dimensional(3-D), biodegradable, acellular scaffold from nativeHUV tissue. Histological examination and scanning electronmicroscopy indicated that native cells in the HUV were removedwith a fine 3-D structure of proteins and proteoglycans wellpreserved. Primary human vocal fold fibroblasts were cultivatedon the abluminal surface of the acellular scaffold in vitro.Significant proliferation and infiltration of the fibroblasts in thescaffold were observed. These findings supported thebiocompatibility of the HUV scaffold, and its promise for vocalfold reconstruction and regeneration.
Roger W.Chan
Otolaryngology-Head & Neck Surgery Graduate Program in Biomedical Engineering University of Texas Southwestern Medical Center Dallas, Texas 75390-9035, U.S.A.
国际会议
成都
英文
1-2
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）