Chondrogenic differentiation of MSCs induced via Cbfa1 driven by SOX9 promoter

摘要：

The good seed cells were the key componentof cartilage tissue engineering. Because of their ownadvantages, mesenchymal stem cells (MSCs) became a goodchoice of seed cells in cartilage tissue engineering field.However, how to regulate MSCs to directly differentiateinto chondrocytes was one of the difficulties at present.Sox9 and Cbfa1 were two important transcription factorsfor chondrogenic differentiation of MSCs and played animportant role in the cartilage formation. In this study, theeukaryotic expression vector of Cbfa1 driven by Sox9promoter was constructed and then was transfected intoMSCs. The higher capacity of stable transfectants(Cbfa1-MSCs) for chondrogenic differentiation had beenidentified. As a readily available source of seed cells forcartilage tissue engineering, Cbfa1-MSCs should havebroad application prospects.

作者: Shiwu Dong Bo Yang Dajun Ying Zhao Xie

作者单位: Biomechanics laboratory, Department of Anatomy,Third Military Medical University,Chongqing, 400038 C Department of Orthopaedics, Southwest Hospital,Third Military Medical University,Chongqing, 400038,

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）