RESEARCH ON SPECROSCOPY OF Cu(Π ) ON PEROXYNITRITE NITRIFYING FIBRINOGEN

摘要：

Many studies have showed that peroxynitrite-mediatedinjury on fibrinogen has some relations with Thromboticdiseases. However there are few reports about structural changesof nitrified fibrinogen when metallic ions exist in this reaction. Inthis study, we use methods of FTIR-ATR spectra and 3DFluorescence spectra to study the structural changes onfibrinogen after nitration and oxidation damages caused byONOO -with Cu(Π) exist. Our results showed that Cu(Π)enhance the production of 3-nitrotyrosine in proteins, The FTIRspectra indicated that the α -helix in fibrinogen reacted withONOO-by adding Cu(Π) decreases from 38.03 % to 28.5 %,whereas β -sheet increases from 29.89% to 43.99 %.Consequently, Cu(Π) could promote Fg nitration with itsconcentration dependence.

作者: YUNJING LUO QI ZHU SHUANG CUI DAWEI ZHENG JIANGNAN SUO

作者单位: College of Life Science and Bioengineering,Beijing University of Technology,Beijing, 100124, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）