Expression of AQP1 in Vascular Endothelia Cells during Angiogenesis is Associated with MRTF-A
Recent studies have demonstrated that the expressionof aquaporin1 (AQP1) in endothelia cells played important rolesin cell migration, and migration-associated cell function such aswound healing, and neutrophil motility. In the present study, weprovided evidence that AQP1 expression in vascular endotheliacells (VECs) during angiogenesis was associated with MRTF-A.Morphology and immunofluorescence assay demonstrated thatVECs from normal and angiogenesis tissues had similar(RT-PCR), Immunoblot and Immunofluorescence analysisidentified that AQP1 expression was significantly higher inangiogenesis VECs than in normal VECs. MRTF-A expressionwas also remarkably enhanced in angiogenesis VECs ascompared to normal VECs, assessed by RT-PCR. Thebioinformatics analysis found that the CarG element existed inthe promoter region of AQP1 gene of many familiar mammals,including of mouse, rat, human etc. These results were the first toindicate that AQP1 was a target gene which could be regulatedby MRTF-A/SRF.
Yong Jiang Zhe Sun Meng-meng Qiang Rui Su Yu-ting Guo Xue-gang Luo Nan Wang Tong-cun Zhang
Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin, China Tianjin Key Laborat College of Biotechnology, Tianjin University of Science and Technology, Tianjin, China Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin, China Tianjin Key Laborat
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）