Optimization of culture conditions for production of Astaxanthin by Phaffia rhodozyma
The effect of medium components (carbon andnitrogen sources) and environmental factors (initial pH,temperature, and working volume) on biomass and astaxanthinproduction in Phaffia rhodozyma was investigated. The mostsuitable carbon, nitrogen, working volume, temperature, andinitial pH for astaxanthin production were sucrose, yeast extract,30ml/500ml, 20 ℃, and pH 7.0, respectively. Subsequently, theoptimum incubation conditions (sucrose 40 g/l, mixed nitrogencontaining yeast extract and peptone 8g/l, working volume 30ml/500ml, and initial pH 9.0) were determined with the orthogonalmatrix method. After 72h fermentation under the optimalincubation conditions, the maximum yield of astaxanthin was 7.83g /l (0.702 mg/g dry cell weight), which were 1.6 fold (0.66 fold)higher than that with a basal medium.
Xuewu Guo Xianyu Li Dongguang Xiao
Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin Industrial Microbiology Key Lab, College of Biotechnology, Tianjin University of Science and Technology, Box08, the 13 th street, TDEA, Tianjin 300457, P.R.China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）