Effect of Cultivating Conditions on γ -Aminobutyric Acid Production by Lactobacillus brevis

摘要：

To investigate the optimum culture conditions ofγ-aminobutyricacid (GABA) production by a strain CC-W11identified as Lactobacillus brevis in submerged fermentation,certain fermentation parameters involving inoculum volume ofseeds, incubation temperature, initial pH value, period ofcultivation and glutamate concentration in medium wereinvestigated separately. The optimal cultivating conditions ofGABA production in submerged fermentation were MRSmedium with 5% monosodium glutamate (MSG), 32℃incubation temperature, 5% inoculum volume, 5.5 initial pH ofmedium, and 60 hours cultivation period. Under the optimizedcultivation conditions, the maximum GABA production was 3.67±0.23 g /L after 60 h of fermentation. This study also showedthat industrial residues from seasoning corporation whichcontained approximately 400~450 g/L MSG could be used byLactobacillus brevis as the substrate of glutamate decarboxylasefor GABA biosynthesis and it was benefit to waste recycling.

作者: Weiqing CHEN Xiaofeng WANG

作者单位: College of Biological and Environmental Engineering Zhejiang Shuren University Hangzhou, China College of Pharmaceutical Science Zhejiang University of Technology Hangzhou, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-3

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）