Preliminary Study on Repeatability of Molecular Recognition Capability of a Silica-Based Molecularly Imprinted Polymer
Molecular imprinting, a templated polymerizationtechnique, is a promising method to prepare a functional polymericnetwork for targeted binding of molecules in a selective recognitionprocess. In this work, a silica-based (polysiloxane) polymer wasutilized in a sol-gel process to fabricate a molecularly imprintedpolymer (MIP). The manufactured MIP was imprinted with atemplate molecule, lysozyme. To test this MIPs molecularrecognitive function, the template molecule lysozyme was extractedfirst; then lysozyme and a competitive molecule, Rnase A, were re-bound;then lysozyme and Rnase A were released and re-bound for a second time in order to evaluate the recognition repeatability of the MIP. Analysis of the repeated testing results implies that thetemplate molecules conformation and affinity played a great rolein affecting the recognition accuracy and efficiency.
Shuang Yu David A Puleo Ai-qin Luo
School of Life Science Beijing Institute of Technology Beijing 100081, China Center for Biomedical E Center for Biomedical Engineering University of Kentucky Lexington, KY 40506, USA School of Life Science Beijing Institute of Technology Beijing 100081, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）