Effect of Surface Modification for Protein Microarray on Immobilizing Probe

摘要：

Glass slides were modified with (3-aminopropyl)-trimethoxysilane, glutaraldehyde, poly-L-lysine to fabricateprotein microarrays. Evaluate and choose more optimal surfacemodification methods for the preparation of protein microarray.The proteins probe labeled by Cy3 was immobilized onto theactivated glass slides, attachment rate was evaluated.Subsequently, the mouse IgG were printed on the modified glassslides, the target proteins were labeled by Cy3, responsiveactivity of protein on microarrays were evaluated. Theattachment rate and the effects of immobilized proteins on theglass were compared. The result showed that the aldehydemodification is more suitable surface modification for thefabrication of protein microarrays.

作者: Tao Wang Rui Zhang Ke-li Xing Zi-yu Guo Li-li Nie Yun-fang Jia

作者单位: Tianjin Medical University Tianjin, P.R.China Nankai University Tianjin, P.R.China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）