An α -amylase biosensor with 1,1-dimethyl-3-(2-amino-1-hydroxyethyl) ferrocene as an electron transfer mediator
A new disposable α -amylase biosensor has been developedbased on the encapsulation of both the glucose oxidase (GOD) and the3-(2-amino-1-hydroxyethyl) ferrocene (DMAHFc) as anelectron transferring mediator was immobilized on the screen-printedelectrode surface by Nafion. Under optimal conditions this biosensorwas able to detect α -amylase activity in the linear range of 15-980 IUL -1 (R2=0.9523), which was wider than some of other α -amylasebiosensors using the flow-injection-type assay and some of otherbiosensors using the gelatin membrane. Three components DMAHFc,improved the sensitivity and long-term stability of the biosensor. Thelimit of detection of 1.2 IU L -1 was obtained, in comparison todetection limits of 50 IU L -1 obtained by some of other α -amylasebiosensors using the flow-injection-type assay and 17 IU L -1 obtainedby some of other biosensors using the gelatin membrane. The activityof salivary α -amylase can be detected by the biosensor with a fastresponse, high sensitivity, good reproducibility, and long-termstability. So, it is possible to construct a new method to identifiespeoples mental stress conditions conveniently.
Jun Zheng Guoming Xie Changtao Chen
Department of Laboratory Medicine Chongqing Medical University Chongqing, China Department of Electron Engineering Sichuan Chemical Technical College Luzhou Sichuan, China
国际会议
成都
英文
1-5
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）