An Adsorbent for Extracorporeal Elimination of Pathogenic Autoantibodies
This work investigated the potential use of benzoicacid (BA)-based immunoadsorbent, a hydrophobic adsorbent, forautoantibodies selective adsorption therapy. The adsorbent wasassessed through chromatographer and batch system assays interms of adsorption capacity, selectivity for bothpolycloneantibodies in normal human plasma and pathogenicantibodies in patients sera. For human immunoglobulin G (IgG),capacities of >30 mg/mL at 10% breakthrough (pH 7.4, linearflow rate of 212 cm/hr) were regularly achieved without addingsalt. Removal percentage of immunoglobulins (Igs) in plasmasamples was 34.2±4.0%, while that of albumin was less than 3%at the ratio of 5 sample volumes per adsorbent volume.Rheumatoid factors in serum samples from 8 rheumatoidarthritis (RA) patients were eliminated at different degrees aftertreatment by the adsorbent. The results indicated that BA-basedagrose was effective in selectively adsorbing human antibodiesfrom plasma, could thus have a potential ability to eliminatepathogenic autoantibodies in apheresis.
Jun Ren Zhiqian Pi Xue Lin Li Xu Jian Xie Lingyun Jia Yue Ma Yumei Wang
Department of Bioscience and Biotechnology Dalian University of Technology Dalian, China Department of Clinical Laboratory The Second Affiliated Hospital of Dalian Medical University Dalian
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）