Microbubbles Conjoining LyP-1 As a Tumor Probe for Ultrasound Molecular Imaging:Targeting Efficiency Evaluation on Microfluidic Platform
Microbubbles promise not only as effective acousticprobes for ultrasonic molecular imaging,but also as drug carriervehicles by conjuncting to specific antibody or ligand.Microbubbles targeting efficiency to certain cells or molecularsis one of the major challenges of being as the acoustic probes. Inthis study, we developed tumor targeted contrast microbubblesby binding LyP-1 (a cyclic nonapeptide acid peptide) targeted tocancer cell. In addition, we utilized microfluidic technology toevaluate the targeted capability and efficiency of microbubbles totumor cell surface. The system includes 8×8 chambermicrofluidic array and each chamber contains eight micro cellsieves which is semicircular for cell trapping. Assisted with thecontrollable fluid shear stress, the microbubbles targeted to thecell and the corresponding affinity efficiency could bequantitatively evaluated under a florescent microscope. Theresult demonstrated that LyP-1 is an efficient maker to becongjuated with microbubbles as tumor targeting probe.Moreover, this system is shown as a useful low-cost and highefficient in vitro platform for studying ultrasonic molecularimaging , drug-delivery and therapy.
Xiang Li Qiaofeng Jin Chunxiang Jiang Zhanhui Wang Hairong Zheng
Paul Lauterbur Center for Biomedical imaging, Institute of Biomedical and Health Engineering Key Lab CAS/CUHK Research Centre for Biosensors and Medical Devices Key Laboratory of Biomedical informatics
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）