Analysis of SGC-7901 Apoptosis Induced by CSBE and Regulating Gene Expression with Laser Scanning Microscopy and Immunofluorescence Flow Cytometry Technique
The purpose of this article was to observeinteraction between drug and cancer cell with fluorescenceprobes and molecular imaging technology. In order to study theeffect of cell growth and apoptosis morphology of Human gastricadenocarcinoma SGC-7901 induced by n-butanol extract fromCapparis spionosa L. (CSBE), laser scanning microscopy wasemployed to observe apoptosis imaging. In addition, the proteinexpression and mRNA expression of its regulating gene Bcl-2 andBax were detected. Laser scanning microscope imaging showedcells that have bound Annexin V-FITC were in early periodof apoptosis, which will show green staining in the plasmamembrane. The cells that have lost membrane integrity willshow red staining (PI) throughout the nucleus and a halo ofgreen staining (FITC) on the cell surface (plasmamembrane). The necrosis cells were stained by PI only. In thewestern blot test, CSBE could decrease the expression of Bcl-2protein and the rate of inhibition increased as the concentrationof drug rised. The immunofluorescence flow cytometry techniquefound that CSBE could increase the expression of Bax protein ina dose-dependent manner. Thus the rate of Bcl-2/Bax wasreduced. The result of RT-PCR showed that CSBE could down-regulatethe mRNA expression of Bcl-2 gene, and up-regulate themRNA expression of Bax gene, which meant that it was byregulating gene transcription level. Eventually the apoptosis ofhuman gastric cancer SGC-7901 cell is induced throughmitochondrial signal transduction pathway.
Yubin JI Lei YU Shiyong GAO Chenfeng JI Xiang ZOU
Institute of Materia Medica and Postdoctoral Programme Harbin University of Commerce Harbin, China Engineering Reseach Center of Natural Anticancer Drugs Ministry of Education Harbin, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）