Endoplasmic reticulum stress signaling pathways is involved in trichosanthin-induced apoptosis in human cervical cancer cell line Hel a
Endoplasmic reticulum stress(ERS)is a kind of sub-cellularpathological process which result from calciumdisorderliness and protein improper folding. And endoplasmicreticulum stress induced apoptosis is a novel pathway whichdifferent from the mitochondria-initiated intrinsic pathway and the death receptor-triggered extrinsic pathway. Trichosanthin(TCS), a type I ribosome-inactivating protein, induces cell deathin various cell types including several tumor cell lines. However,the mechanism remains largely uncharacterized. The aim ofpresent study is to explore possible mechanism underlying itscytotoxicity by using human cervical cancer Hela cell line as amodel. We found that TCS induced apoptosis in Hela cells in atime-and dose-dependent manner. cDNA microarray analysisdemonstrated that there were 62 pieces of gene up-regulated and16 pieces of gene down-regulated after treated with TCS,subsequent analysis by RT-PCR and western blot confirmed thatGRP78 and calpain could be significantly induced by TCS ,which strongly supported the involvement of endoplasmicreticulum stress pathway in TCS-induced apoptosis. This studyreveals a new pathway of endoplamic reticulum stress in TCSinduces apoptosis in hela cells, Further research with more celllines and calpain inhibitor needs to be performed to determine ifcalpian activation is a common feature in TCS-induced apoptosis.
Yiling Huang Liming Huang Chengcheng You HuoJun Hu
dept.of pathology medical school , China Three Gorges University Yichang , HuBei, China dept.of neurosurgery,the first clinical hospital of China Three Gorges University YiChang , HuBei, C
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）