Quantitative Analysis of DNA Interstrand Crosslink Induced by Chloroethylnitrosoureas with Real-Time Fluorometric Assay

摘要：

Chloroethylnitrosoureas (CENUs) were an importantfamily of anticancer agents in the clinical treatment of humanmalignancies. Several lines of evidences indicated that theircytotoxicity was related to the interstrand crosslink of DNA. Inthis study, a method for the determination of DNA crosslink wasestablished using real-time fluorescence quantitative PCR. TheDNA crosslink ratio induced by two CENUs, Semustine(Me-CCNU) and Carmustine (BCNU) were quantitativelyanalyzed and compared. The results showed that BCNU andMe-CCNU exhibit different crosslink trend of double strand DNA.Evagreen, a fluorescent dye, was used as the probe in the assay.This study provided direct evidence that real-time fluorescencequantitative PCR (qPCR) was a more convenient, efficient andstable method to detect DNA crosslink.

作者: REN Ting ZHAO Li-Jiao TANG Wei ZHENG Da-Wei ZHONG Ru-Gang

作者单位: College of Life Science and Bioengineering, Beijing University of Technology, Beijing, 100124, China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-4

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）