The varifocal scanning Micro-zooming technique: a new method for measuring the neurite length in three dimensional spaces
Neurite length measurement is very important in describing neurons physiological states and of morphological parameters. Combining computer technology and modern microscopic optical technology can develop a high precision measurement for measuring the neurite length in threedimensional space. Through record Zeiss microscope spiral rotation angle in two different planes and Matlabs excellent image process compatibility, the neurite total length and the distance between two adjacent plane can be stored and analysised. Single and connect centerlines alone neurite are extracted. Based on the fast neurite centerlines extracting systems and automatic data processing, we have produced an automated neurite labeling and measuring system that could be used for quantitatively studying neuronal structure in 3D cultures.
Zhang Wei Zheng Xianju Li Jiye Peng Jin Zhang Wei
Computer engineering dept.Chengdu Electromechanical College, CEC Sichuan, Chengdu, P.R.China Histology embryology & neurobiology dept.Sichuan University, SCU Sichuan, Chengdu, P.R.China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）