Purification and identification of soluble Catechol-O-methyltransferase from rat liver
Soluble catechol-O-methyltransferase (S-COMT) is an important target protein due to its involvement in some human disorders. The main scope of the work was to develop an approach to purify and identify S-COMT. Its isoelectric point was 5.0 determined with Offgel. A simplified process including extraction, selective precipitation, ammonium sulfate precipitation, gel filtration chromatography and anion exchange chromatography was established to obtain S-COMT of a certain purity degree. The molecular was 25kD. Mass spectrometry and mascot were employed for further identification.
Lina Yang Jianhui Yan Yulin Deng
School of Life Science and Technology Beijing Institute of Technology Yiling Pharmaceutical Group Be Yiling Pharmaceutical Group Beijing100081, China School of Life Science and Technology Beijing Institute of Technology
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）