The Effects of Estrogen on Cytoplasmic Ca2+ Concentration of Masticatory Muscles Myoblast in Acid Condition
Temporomandibular disorders (TMD) affect women with a higher frequency than men, which suggests a possible link between its pathogenesis and sex hormone, such as estrogen. Cytoplasmic Ca2+plays an important role in cell normal function and cytoplasmic Ca2+accumulation may be a key factor that causes skeletal muscle fatigue and injury. To investigate the effects of estrogen on intracellular Ca2+concentration of masticatory muscles, myoblasts from masticatory muscles of female Sprague-Dawley rats were cultured and loaded with Fluo-4 AM, the indicator of Ca2+. And the dynamic Ca2+transients were visualized with confocal laser-scanning microscopy. 17β-estradiol was added to the myoblasts in resting and acid condition (PH=6.7). In the resting condition, 17β-estradiol with the concentration of 10﹣9mol/L, 10﹣8mol/L and 10﹣7mol/L lead cytoplasmic Ca2+concentration to increase immediately, followed by decreasing right away, and in the end came into a Ca2+homeostasis similar with the concentration at base line. In the acid condition (PH=6.7) which mimicked muscle fatigue state, 17β-estradiol with the concentration of 10﹣9mol/L、10﹣8mol/L and 10﹣ 7mol/L induced the cytoplasmic Ca2+concentration to decrease immediately, then came into a new Ca2+homeostasis which was lower than the concentration at base line. The results suggest that in acid condition, estrogen may maintain the skeletal cytoplasmic Ca2+concentration in a lower level and reduce the cytoplasmic Ca2+accumulation to keep the normal functions of masticatory muscles myoblasts.
Xijun Wang Hongmei Guo Yanmin Wang Xinzhu Yi
Department of Prosthodontics, School of Stomatology, Shandong University Jinan, China State Key Laboratory of Oral Diseases Sichuan University Chengdu, China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）