Preparation of Dextran –PAB Carrier for Affinity Ultrafiltration

摘要：

Affinity ultrafiltration as a new filtration technology has both the advantages of affinity purification and membrane separation. In this study ， the dextran T2000 (dextran with nominal molecular weight of 2 million) was employed， after epichlorohydrin activation and coupled with PAB(P-aminobenzamidine） as affinity ligand，we got affinity ultrafiltration carrier with the ligand concentration of 108.21μmol/g. The changes of infrared adsorption spectrum and ultraviolet adsorption spectrum in the processing of affinity carrier preparing were researched, and pilot study on trypsin purified by the affinity filtration was performed.

作者: Song-Qing Hu Sha-Sha Guo Hai-Liu Wei Ping Chen Yi Hou Xi-Mei Zang Lin Li

作者单位: College of Light Chemistry and Food Science, South China University of Technology, Guangzhou, 510640, P.R.China

会议类型: 国际会议

会议名称: The 4th International Conference on Bioinformatics and Biomedical Engineering(第四届IEEE生物信息与生物医学工程国际会议 iCBBE 2010)

会议地点: 成都

会议语种:英文

页码: 1-3

在线出版日期: 2010-06-18（万方平台首次上网日期，不代表论文的发表时间）