Optimization of conditions for the Biotransformation of 5-Cyanovaleramide from Adiponitrile by Rhodococcus ruber CGMCC 3090
Adiponitrile was regioselectively converted to 5-cyanovaleramide as the single product using resting cells of Rhodococcus ruber CGMCC3090. In this research, the reaction characteristics of regioselective biotransformation of adiponitrile to 5-cyanovaleramide using Rhodococcus ruber CGMCC3090 was studied, and the reaction conditions were optimized. As a result, the optimum reaction temperature and pH were 30℃ and 7.5, respectively. The deactivation energy of the reaction was 20.91 kJ/mol according to Arrhenius equation. Bioconversion capacity was increased by 10% with adding 2.5% (V/V) methanol in the reaction system. When 10-4 mol/L of Co2+was added to the reaction system, the conversion ratio was increased by 40%. However, the enzyme activity was significantly inhibited by 10-4 mol/L of Zn2+or Cu2+. Under optimal conditions, 1.5 mol/L adiponitrile was converted to 5-cyanovaleramide with the cell concentration of 0.88 g/L, with the transformation ratio and regioselectiverty of both 100%.
Xiao-Dan Li Min Wang Yu Zheng Jian-mei Luo Hua Sun
Key Laboratory of Industrial Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science and Technology, Tianjin 300457, P.R.China
国际会议
成都
英文
1-4
2010-06-18（万方平台首次上网日期，不代表论文的发表时间）